发明名称 |
IN VITRO MICROPHYSIOLOGICAL SYSTEM FOR HIGH THROUGHPUT 3D TISSUE ORGANIZATION AND BIOLOGICAL FUNCTION |
摘要 |
Techniques for generating microtissues, including a micro-fabricated platform including at least one micro-well including a plurality of micro-cantilevers coupled thereto and surrounded by a plurality of ridges, each micro-cantilever including a cap at a terminal end thereof. The platform can be immersed in a suspension of cells. The suspension of cells can be driven into at least one micro-well, and the ridges can be de-wetted to remove excess suspension and isolate the suspension of cells in each micro-well. The cells can be driven in the suspension of each micro-well toward a top surface of the suspension, which can be polymerized to form a matrix. The cells can be cultivated to spontaneously compact the matrix such that the micro-cantilevers anchor and constrain the contracting matrix to form a band of microtissue that spans across the micro-cantilevers. |
申请公布号 |
EP2766473(A4) |
申请公布日期 |
2015.03.11 |
申请号 |
EP20120839742 |
申请日期 |
2012.10.12 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
CHEN, CHRISTOPHER, S.;MARGULIES, KEN;BOUDOU, THOMAS;LEGANT, WESLEY;YANG, MICHAEL, T. |
分类号 |
C12N5/00;C12M1/32;C12M1/34;C12M3/00;C12N1/14;C12N1/20;C12N5/077;C12N7/00;C12N11/00;C12Q1/02;C12Q1/68;G01N33/50 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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