Compositions and methods for the therapy and diagnosis of influenza

摘要

The present invention provides compositions, vaccines, and methods for diagnosing, treating, and preventing influenza infection using a combination of antibodies raised against the influenza hemagglutinin and the matrix 2 ectodomain polypeptides.

主权项

1. A composition comprising:
(a) an isolated human monoclonal antibody that specifically binds to an epitope of the hemagglutinin (HA) glycoprotein of an influenza virus, wherein said antibody comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 435 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 438; and (b) an isolated human monoclonal antibody that specifically binds to an epitope in the extracellular domain of the matrix 2 ectodomain (M2e) polypeptide of an influenza virus, wherein said antibody comprises a VH CDR1 region comprising the amino acid sequence of NYYWS (SEQ ID NO: 72); a VH CDR2 region comprising the amino acid sequence of FIYYGGNTKYNPSLKS (SEQ ID NO: 74); a VH CDR3 region comprising the amino acid sequence of ASCSGGYCILD (SEQ ID NO: 76); a VL CDR1 region comprising the amino acid sequence of RASQNIYKYLN (SEQ ID NO: 59); a VL CDR2 region comprising the amino acid sequence of AASGLQS (SEQ ID NO: 61); a VL CDR3 region comprising the amino acid sequence of and QQSYSPPLT (SEQ ID NO: 63), and wherein said epitope of the M2e polypeptide is a discontinuous epitope.