Multiwell plate lid for improved optical measurements

摘要

A lid for multiwell plates, allowing improved optical measurement of liquid samples within its wells, while mitigating evaporation from said samples, is disclosed. A surface element protrudes from the bottom of the lid into the fluid within a well. The protruding element may be hollow or solid such that light directed into the element may act to capture or direct the beam while preventing backscatter from reaching one or more detectors. The protruding element may direct the beam from the well without requiring the beam to pass through a fluid/air interface. The angle and shape of the lid surfaces and/or light absorbing/blocking colorization may be employed to minimize or eliminate back reflection. Evaporation is controlled by physically capping the well with the lid, either sealing against the face at the top of the well or the inside surface of the well.

主权项

1. A multi-well plate lid, said lid being a removable or hinged cover for the top of said multi-well plate, for reducing evaporation from, while improving optical measurements of a plurality of liquid samples within the wells of a multiwell plate comprising
A) a top surface; B) a bottom surface which rests on, or is seated above, the surface surrounding the openings of said sample containing wells; and C) a plurality of elements protruding from said lid bottom surface, each of said elements extending into an individual liquid sample containing well, wherein said protruding elements are arranged such that they may convey a light beam passing through said sample from below or entering said sample through said elements, wherein said protruding elements comprise solid posts which comprise
a. an inner core comprising at least one a flat surface which is intersected by said light beam, said inner core being transparent or partially transparent at the wavelength of said light beam; andb. an outer circumferential surface which absorbs light at the wavelength of said light beam.