Methods for dispersing somatic plant embryos

摘要

Methods and devices for dispersion of clusters of somatic plant embryos suspended in a liquid are disclosed. The methods comprise i) subjecting the clusters of embryos to fluid dynamics forces causing axially extensional strain and radially compressional strain and ii) subjecting the clusters of embryos to fluid dynamics forces causing axially compressional strain and radially extensional strain fluid dynamics and iii) repeating said steps in sequence until the individual embryos are separated from each other. The devices may comprise a flow channel including at least one constriction, such that clusters of embryos flowing through the flow channel are first subjected to axially extensional strain and radially compressional strain, and then to axially compressional strain and radially extensional strain from fluid dynamics forces.

主权项

1. A method of dispersion of clusters of plant embryos suspended in a liquid into individual plant embryos flowing through a flow channel, the method comprising:
feeding clusters of plant embryos into a disperser for separating individual plant embryos contained in the clusters of embryos; subjecting the clusters of plant embryos to axially extensional strain and radially compressional strain; subjecting the clusters of plant embryos to fluid dynamics forces causing axially compressional strain and radially extensional strain from fluid dynamics forces; and repeating the subjecting steps in sequence until the individual plant embryos are separated from each other, wherein the disperser comprises a flow channel including at least two constrictions, wherein each constriction of the at least two constrictions has a cross-sectional area in the interval from 0.75 to 1300 mm2, wherein the flow channel includes an intermediate portion between sequential constrictions, wherein the minimum cross-sectional dimension of the flow channel is larger than the largest dimension of the largest part of an individual plant embryo in the clusters of plant embryos to be dispersed, and wherein the longitudinal axes of said at least two constrictions align with each other.