Microorganisims used to synthesize oligosaccharides

摘要

The present invention relates to the enzymatic synthesis of oligosaccharides, particularly, sialylated oligosaccharides comprising the carbohydrate moeities of the gangliosides GM3, GD3, and GT3.

主权项

1. An Escherichia coli microorganism comprising a heterologous gene encoding a bifunctional Campylobacter jejuni sialyltransferase polypeptide that catalyzes the transfer of a sialyl moiety from an activated sialic acid molecule to lactose to form Neu5Acα-3Galβ-4Glc, wherein the Escherichia coli microorganism lacks Neu5Ac aldolase activity and β galactosidase activity, and wherein the heterologous gene encoding a bifunctional Campylobacter jejuni sialyltransferase has at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, and 4, and wherein the microorganism is LacY+ and NanT+ allowing active transport of lactose and sialylic acid.