Mutant RpsA gene and method for producing L-amino acid

摘要

A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided. A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at position 210 is replaced with another amino acid residue is used. This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.

主权项

1. A method for producing an L-amino acid, the method comprising:
A) culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability in a medium containing a carbon source selected from the group consisting of a fatty acid and an alcohol; and B) collecting the L-amino acid from the medium, wherein the bacterium expresses an ribosomal protein S1 RpsA protein comprising the amino acid sequence of SEQ ID NO: 2, wherein said RpsA protein has a mutation in which the aspartic acid residue at position 210 of SEQ ID NO: 2 is replaced with a different amino acid residue, and wherein said bacterium is Escherichia coli.