Tissue Culture Chip

摘要

A dual chamber bioreactor for producing complex, multilayer tissue, organs, organ parts, and skin replacements has been developed. The bioreactor is modular and incorporates a removable tissue culture cassette. By rotating the dual chamber bioreactor along the horizontal axis, different populations of cells with different growth requirements can be cultured on the different surfaces of the tissue culture cassette that are exposed to different media reservoirs. Culturing different populations of cells on different surfaces of the tissue culture cassette enables the production of multilayer tissue and organs. The tissue culture cassette can contain one or more discrete tissue culture sections.

主权项

1. A tissue culture cassette comprising a first and second gasket, wherein the first and second gaskets each comprise an opening that aligns with the opening of the other when the first and second gaskets are combined, and
a cell culture matrix or scaffold sandwiched between the first and second gasket that covers the aligned openings to form an impermeable tissue culture cassette.