| 主权项 |
1. A method for identifying, from a nucleic acid library, a nucleic acid encoding an antigen having the ability, either alone or in combination with one or more other antigens, to stimulate an anti-cancer response, wherein said nucleic acid library is a library of virally-expressed nucleic acid members and has the ability to stimulate an anti-cancer response when administered to a mammal having cancer, wherein said method comprises:
(a) contacting immune cells with an aliquot of said nucleic acid library to form a collection of virally infected cells, wherein said immune cells were obtained from a mammal that exhibited a reduction in the number of cancer cells following administration of a first nucleic acid library, (b) obtaining a cell from said collection of virally infected cells that has the ability to secrete a cytokine polypeptide, wherein said cell contains a nucleic acid member of said nucleic acid library, and (c) determining the sequence of said nucleic acid member of said cell, wherein said nucleic acid member is said nucleic acid encoding an antigen having the ability, either alone or in combination with one or more other antigens, to stimulate an anti-cancer response. |
