Transgenic Plants And A Transient Transformation System For Genome-Wide Transcription Factor Target Discovery

摘要

Plant genes regulated by transcription factors that control the gene network response to an environmental perturbation or signal are described. This class of genes responds to the perturbation of a transcription factor and the signal it transduces, but surprisingly, without stable binding of the transcription factor. These genes represent members of the “dark matter” of metabolic regulatory circuits.;The invention involves the transgenic manipulation of these “response genes” and/or the genes encoding their regulatory transcription factors in plants so that their respective gene products are either overexpressed or underexpressed in the plant in order to confer a desired phenotype.;The invention also relates to a rapid technique named “TARGET” (Transient Assay Reporting Genome-wide Effects of Transcription factors) for determining such “response genes” and their transcription factors by perturbation of the expression of the transcription factors of interest in protoplasts of any plant species.

主权项

1. A transgenic plant that ectopically expresses one or more hit-and-run transcription factor genes and exhibits a desired phenotype, wherein the said one or more genes comprises a polynucleotide that encodes At1g01060, At1g01720, At1g13300, At1g15100, At1g22070, At1g25550, At1g25560, At1g29160, At1g43160, At1g51700, At1g51950, At1g53910, At1 g66140, At1 g68670, At1 g68840, At1 g74660, At1 g74840, At1 g75390, At1 g77450, At1g80840, At2g04880, At2g20570, At2g22430, At2g22850, At2g24570, At2g25000, At2g28510, At2g28550, At2g30250, At2g33710, At2g38470, At2g46830, At3g01560, At3g04070, At3g06590, At3g20770, At3g25790, At3g46130, At3g47620, At3g51920, At3g54620, At3g60490, At3g61150, At3g61890, At3g62420, At4g17490, At4g17500, At4g24240, At4g27410, At4g31800, At4g34590, At4g36540, At4g37180, At4g37260, At4g37610, At4g37730, At5g05410, At5g06800, At5G10030, At5g13080, At5g14540, At5g24800, At5g39610, At5g44190, At5g47230, At5g48655, At5g49450, At5g49520, At5g56270, At5g60850, At5g63790, At5G65210, or At5g65640.