SINGLE STEP FRACTIONATION METHOD

摘要

Single step method of enriching highly sialylated variant/s of a protein by use of a strong ion exchange chromatography support and a pH gradient in absence of any salt gradient.

主权项

1. A method for fractionation of highly sialylated variants of an erythropoiesis stimulating protein comprising:
a) loading a clarified cell culture broth comprising a mixture of differentially sialylated erythropoiesis stimulating protein on to an ion exchange resin, and b) eluting the bound protein using a pH gradient at a constant salt concentration, wherein the eluate is enriched in highly sialylated variants of the protein.