TUNING MICROBIAL POPULATIONS WITH PROGRAMMABLE NUCLEASES

摘要

Various aspects and embodiments of the invention are directed to methods and compositions for reversing antibiotic resistance or virulence in and/or destroying pathogenic microbial cells such as, for example, pathogenic bacterial cells. The methods include exposing microbial cells to a delivery vehicle with at least one nucleic acid encoding an engineered autonomously distributed circuit that contains a programmable nuclease targeted to one or multiple genes of interest.

主权项

1. A method, comprising delivering to microbial cells in vivo a delivery vehicle with at least one nucleic acid encoding an engineered autonomously distributed circuit that contains a programmable nuclease that targets a gene or nucleotide sequence of interest.