CELL CULTURE MEDIA AND METHODS

摘要

Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

主权项

1. A method of making a eukaryotic cell culture medium, feed or supplement, the method comprising:
(i) adding a minimal volume of an aqueous solution to a dry powder format of the medium, feed or supplement to make a paste; (ii) mixing the paste vigorously to prepare a microsuspension.