IMMUNOHISTOCHEMICAL ASSAY FOR DETECTION OF CD3 AND CD16

摘要

The present application provides methods and systems for detecting both CD3 and CD16 in a sample using immunohistochemical methods, for example when the primary antibodies for CD3 and CD16 are from the same species (e.g., both rabbit antibodies). Methods to denature and block the first primary antibody contacted with the sample are provided.