FLUORESCENCE MICROTITRE PLATE READER

摘要

A fluorescence microtitre plate reader comprises a plate reader body (2) having a base (3), top (5) and sidewalls (4), the top of the plate reader comprising at least one seat (6) dimensioned for receipt of a microtitre plate (7), the at least one seat defining an aperture (8) in the top of the plate that substantially corresponds to a base of the plate. The plate reader also includes means for illuminating wells of a microtitre plate with light from beneath the plate having an excitation wavelength, and a camera (10) and lens (11) assembly for capturing light emitted by the wells of the microtitre plate and generating a digital image, in which regions of light intensity of the image correspond to wells of the microtitre plate. An optical system is disposed within the plate reader body for expanding the distance to image plane of light, the optical system comprising a first mirror (15) disposed to receive light from the wells of the microtitre plate and reflect the light to second mirror (16) which is disposed to reflect incident light to the camera lens. The means for illumining the wells of the microtitre plate comprises a ring light (12) that embraces the camera and lens assembly.

主权项

1. A microtitre plate reader for detecting fluorescence of a fluorescent dye in the wells of a microtitre plate, the microtitre plate reader comprising:
a plate reader body (2) having a base (3), top (5) and sidewalls (4), the top of the plate reader body comprising at least one seat (6) dimensioned for receipt of a microtitre plate (7), the at least one seat defining an aperature (8) that substantially corresponds to a base of the plate; means for illuminating wells of a microtitre plate with light from beneath the plate; a camera (10) and lens (11) assembly for capturing light emitted by the wells of the microtitre plate and generating a digital image, in which regions of light intensity of the image correspond to wells of the microtitre plate; and an optical system disposed within the plate reader body for expanding the distance to image plane of light and comprising at least two mirrors (15, 16),characterised in that the means for illuminating the wells of the microtitre plate comprises a ring light (12) that embraces the camera and lens assembly.