SYNTHETIC OLIGONUCLEOTIDES FOR DETECTION OF NUCLEIC ACID BINDING PROTEINS

摘要

Synthetic oligonucleotides that comprise a nucleic acid binding protein binding site, PCR primer sequences, and tag sequences that do not bind to nucleic acid binding proteins, with a total length of 85-130 nucleotides are disclosed herein. Also disclosed are libraries and kits comprising the synthetic oligonucleotides as well as methods of detecting nucleic acid binding proteins in a sample using the synthetic oligonucleotides.

主权项

1. A synthetic oligonucleotide comprising:
a first sequence comprising a nucleic acid binding protein binding site; a second sequence comprising a binding site for a first PCR primer or an RNA polymerase promoter; a third sequence comprising a tag sequence, wherein the tag sequence binds no known nucleic acid binding protein; wherein at least the first sequence is double stranded and wherein the total length of the oligonucleotide is between 85 and 130 nucleotides.