发明名称 |
COMPOSITIONS FOR LINKING DNA-BINDING DOMAINS AND CLEAVAGE DOMAINS |
摘要 |
Disclosed herein are compositions for linking DNA-binding domains and cleavage domains to form nucleases, for example nucleases with altered target site separation (gap) preferences as compared to conventional linkers. Also described are fusion proteins comprising these linkers. The disclosure also provides methods of using these fusion proteins and compositions thereof for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined region of interest in cells. Thus, in one aspect, described herein are amino acid sequences linking a DNA-binding domain to a cleavage domain. The linker may replace one or more residues of the DNA-binding domain and/or cleavage domain. |
申请公布号 |
WO2015031619(A1) |
申请公布日期 |
2015.03.05 |
申请号 |
WO2014US53170 |
申请日期 |
2014.08.28 |
申请人 |
SANGAMO BIOSCIENCES, INC. |
发明人 |
PASCHON, DAVID;ZHANG, LEI |
分类号 |
C12N9/16 |
主分类号 |
C12N9/16 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|