AMPEROMETRIC DETECTION OF LIMULUS AMEBOCYTE LYSATE ACTIVATION BY ENDOTOXIN AND/OR 1-3-BETA-D-GLUCAN

摘要

Devices to detect detecting endotoxin (lipopolysaccharide or LPS) and/or 1-3-β-D-glucan (beta glucan or BG) using substrates including electrogenic mediators for amperometric detection are provided herein. Substrates include an amine group as well as one or more substituted organic rings, such as a phenol. Devices include cartridges for detecting LPS or BG simultaneously using dual detection techniques.

主权项

1. An electrochemical substrate for detecting endotoxins and/or 1-3-β-D-glucan, the substrate comprising:
a peptide, and an electrochemical group selected from one of the following structures: wherein R1 is a primary amine, or a secondary amine, R2 is a hydroxyl, a methoxy, an ether, a ketone, an aldehyde, or a carboxylic acid, R3 is a hydrogen, halide, or hydroxyl, R4 is a hydrogen or hydrocarbon chain R is a hydrogen or hydrocarbon chain, and R5 is a methoxy, an ether, a ketone, an aldehyde, or a carboxylic acid.