| 摘要 |
1,208,226. L-Asparaginase. KYOWA HAKKO KOGYO CO. Ltd. 24 April, 1969 [24 May, 1968], No. 20971/69. Heading C3H. In a process for purifying an enzyme preparation of L-asparaginase obtained from an L- asparaginase-producing micro-organism belonging to the genus Serratia, wherein said microorganism produces together with the L-asparaginase factors which inactivate the enzymatic activity of L-asparaginase, the inactivating factors are separated from L-asparaginase during some stage of the purification process by salting out with the addition of an inorganic salt, and purified L-asparaginase having an effective anti-tumour activity is recovered. The salt used may be sodium chloride or ammonium sulphate. The solution of crude enzyme may first be treated with manganese ions to remove nucleic acids. The solution may be treated by chromatography on CM-cellulose or DEAE-cellulose before or after the salting-out of inactivating factors.
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