Rapid electrophoresis binding method and related kits and compositions

摘要

An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described.

主权项

1. An integrated method for staining a target molecule with a staining reagent, the method comprising: moving the target molecule into a matrix by applying a first electric force to the target molecule to obtain a matrix embedded with the target molecule; moving the staining reagent into the matrix embedded with the target molecule by applying a second electric force to the staining reagent, and moving excess free staining reagent out of the matrix by applying the second electric force to the excess free staining reagent, wherein the staining reagent stains the target molecule in the matrix, and the first electric force is transverse to the second electric force.