Analysis of amino acids in body fluid by liquid chromatography-mass spectrometry

摘要

This disclosure provides methods for quantifying individual amino acids in various bodily fluids obtained from a human patient. Also provided are reference ranges for normal amino acid levels in the various bodily fluids (e.g., blood plasma, urine, cerebrospinal fluid, and saliva) and for various age groups (e.g., neonates, infants, children, and adults).

主权项

1. A method for determining the amount of one or more free amino acids in a body fluid of an individual by mass spectrometry, the method comprising:
(a) separating the one or more free amino acids by liquid chromatography (LC); and (b) measuring the amount of the one or more free amino acids by mass spectrometry (MS) analysis; wherein the one or more free amino acids comprise at least one amino acid selected from phosphoserine, allo-isoleucine, and sulfocysteine.