发明名称 |
Method for recovering sperm nucleic acid from a forensic sample |
摘要 |
A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided. |
申请公布号 |
US8969044(B2) |
申请公布日期 |
2015.03.03 |
申请号 |
US200812177579 |
申请日期 |
2008.07.22 |
申请人 |
Life Technologies Corporation |
发明人 |
Liu Jason Yingjie |
分类号 |
C12P19/34;C12N15/10 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
1. A method for selectively recovering DNA from sperm cells from a sample containing intact sperm cells and intact epithelial cells, the method comprising the steps of:
(a) washing a sample comprising intact sperm cells and intact epithelial cells by mixing the sample with a washing solution, wherein the washing solution comprises from 30 mM to 260 mM MgCl2 and thereby forming a washed sample; (b) applying DNase to the washed sample and thereby forming a DNased sample; (c) applying a selective sperm lysis buffer comprising NaCl and DTT to the DNased sample of step (b); and (d) isolating sperm DNA from the sample of step (c). |
地址 |
Carlsbad CA US |