Alkynyl-derivatized cap analogs, preparation and uses thereof

摘要

Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.

主权项

1. A composition comprising an alkynyl-derivatized cap analog having the structure:
R37,3′-O-alkynylG[5′]p[p]np[5′]G, R37,3′-O-alkynylG[5′]p[p]np[5′]A, R37,2′-O-alkynylG[5′]p[p]np[5′]G, R37,2′-O-alkynylG[5′]p[p]np[5′]A, or a salt thereof,
wherein
R3 is alkyl or arylalkyl,the alkynyl moiety comprises 3-24 carbon atoms, a terminal alkyne, and is optionally substituted,n is 1, 2, or 3,A is adenosine, andG is guanosine.