Methods and compositions for diagnosis and prognosis of renal injury and renal failure

摘要

Disclosed is a method for evaluating renal status in a subject, comprising: - performing a plurality of assays configured to detect both of the proteins metalloproteinase inhibitor 2 and neutrophil elastase in one or more body fluid samples obtained previously from the subject by contacting the body fluid sample(s) with molecules that bind to each of metalloproteinase inhibitor 2 and neutrophil elastase under conditions sufficient for such binding to occur; - determining a measured concentration of each of metalloproteinase inhibitor 2 and neutrophil elastase from the amounts of the molecules bound thereto in the body fluid samples; - combining the measured concentration of each of metalloproteinase inhibitor 2 and neutrophil elastase using a function that converts the assay results into a single composite result to thereby provide a combined concentration of metalloproteinase inhibitor 2 and neutrophil elastase for the subject; - comparing the combined concentration of metalloproteinase inhibitor 2 and neutrophil elastase to a threshold combined concentration; and correlating the assay results to the renal status of the subject, said correlating comprising: (i) assigning a diagnosis that reduced or worsening renal function or an event an event associated with reduced or worsening renal function has occurred, or assigning a risk or likelihood that reduced or worsening renal function or an event an event associated with reduced or worsening renal function is likely to occur in future, when the combined concentration of metalloproteinase inhibitor 2 and neutrophil elastase is above the threshold concentration; or (ii) assigning a diagnosis that reduced or worsening renal function or an event an event associated with reduced or worsening renal function has not occurred, or assigning a risk or likelihood that reduced or worsening renal function or an event an event associated with reduced or worsening renal function is not likely to occur in future, when the combined concentration of metalloproteinase inhibitor 2 and neutrophil elastase is below the threshold concentration.