Compositions and methods of producing enterokinase in yeast

摘要

Disclosed is an isolated polynucleotide molecule comprising the nucleotide sequence of SEQ ID NO: 4 or SEQ ID NO: 6; wherein the sequences are as defined in the specification. Also disclosed is a yeast expression construct comprising a yeast expression vector operably-linked to a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 4. Further disclosed is a method of producing an enterokinase, the method comprising the step of culturing a yeast cell transformed with a yeast expression construct comprising a polynucleotide haying the nucleotide sequence of SEQ ID NO: 4 or SEQ ID NO: 6 to thereby produce an enterokinase encoded by the nucleotide sequence of SEQ ID NO: 4 or SEQ ID NO: 6.