METHOD OF BIOLOGICAL LIGAND PRINTING DURING BIOCHIP PRODUCTION

摘要

FIELD: biotechnologies.SUBSTANCE: invention offers method of printing biological ligands in the form of oligosaccharides and/or polysaccharides, and/or peptides, and/or glycopeptides, and/or biotin. A layer of hydrophobic non-volatile fluid not mixing with biological ligand solvent and having lower specific weight than biological ligand solution is applied in advance onto a substrate. Then biological ligands are printed by contact-free method. Vaseline, mineral oil or higher saturated hydrocarbons or mix thereof are used as hydrophobic non-volatile fluid. Preferable thickness of hydrophobic nonvolatile fluid layer is 50-200 mcm.EFFECT: more level drying of biological ligand solution drops on substrate along with protection of biological ligands against early hydrolysis and improvement of spot morphology.3 cl, 2 dwg, 3 ex