| 摘要 |
Disclosed is a method for producing antibodies specific for an antigen of interest comprising: a) selecting a B-cell capable of producing antibody specific for said antigen of interest or selecting a B-cell capable of differentiating into a B-cell which is capable of producing antibody specific for said antigen of interest; b) inducing, enhancing and/or maintaining expression of BCL6 in said B-cell; c) inducing, enhancing and/or maintaining expression of an anti-apoptotic nucleic acid in said B-cell; d) allowing expansion of said B-cell into a population of said B-cells; e) selecting at least one B-cell from said population of B-cells producing a B-cell receptor and/or antibody with a binding capacity higher than the average binding capacity of said population of B-cells for said antigen of interest; f) culturing said at least one B-cell into a population of B-cells; and g) obtaining antibodies produced by the B-cell culture. |
