发明名称 |
Oligonucleotide Primer with Omega Structure for Detecting Short-Chain RNAs and Use Thereof |
摘要 |
Disclosed are an oligonucleotide primer with an omega structure for detecting short-chain RNAs and the use thereof. The primer from the 5′ end to the 3′ end sequentially is: a PCR primer target region of 20-30 bases, a variable coding region of 0-50 bases, an omega stem-loop, a probe spacer of at least one base and a probe region of 4-11 bases. The length of the stem of the omega stem-loop is 4-12 paired bases, and the length of the loop of the omega stem-loop is 3-20 unpaired bases. |
申请公布号 |
US2015056623(A1) |
申请公布日期 |
2015.02.26 |
申请号 |
US201314373305 |
申请日期 |
2013.01.16 |
申请人 |
Chengdu Nuoen Biological Technology Co., LTD |
发明人 |
Zhang Songbo;Jiang Guobiao;Tang Fang;Zhang Feifei |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. An oligonucleotide primer with omega structure for short-chain RNAs detection, characterized in that: said primer has a 5′ terminal through a 3′ terminal sequentially comprising: a PCR primer target region, a variable coding region, an omega stem-loop, a spacer region for probe and a probe region, wherein a length of said PCR primer target region is 20-30 bases, a length of said variable coding region is 0-50 bases, a stem length of said omega stem-loop is 4-12 bases in pair and said bases in pair and has a cis-structure, a loop length of said omega stem-loop is 3-20 bases in non-pair alignment, a length of said spacer region for probe is at least 1 base, and a length of said probe region is 4-11 bases. |
地址 |
Chengdu CN |