METHODS FOR RAPID IDENTIFICATION OF PATHOGENS IN HUMANS AND ANIMALS

摘要

The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples.

主权项

1. A method of identifying a plurality of etiologic agents of disease in an individual comprising the steps of:
amplifying at least one nucleic acid molecule obtained from a biological sample from the individual with a plurality of intelligent primers to obtain a plurality of amplification products corresponding to the plurality of etiologic agents; and determining the molecular masses of the plurality of amplification products, wherein the molecular masses identify the plurality of etiologic agents and wherein the intelligent primers are broad range survey primers, division-wide primers, drill-down primers, or any combination thereof.