RECOMBINANT PAPAYA MOSAIC VIRUS COAT PROTEINS AND USES THEREOF IN INFLUENZA VACCINES

摘要

Recombinant papaya mosaic virus (PapMV) coat proteins comprising one or more antigenic peptides derived from an influenza virus antigen, such as from the M2e peptide, fused at a position within a predicted random coil within 13 amino acids of the N-terminus of the coat protein, uses thereof to prepare virus-like particles (VLPs), and uses of the VLPs in influenza vaccines.

主权项

1. A fusion protein comprising a peptide antigen derived from influenza M2e peptide fused to a papaya mosaic virus (PapMV) coat protein after an amino acid that corresponds to amino acid 6, 7, 8, 9, 10, 11 or 12 of SEQ ID NO:1, wherein the fusion protein is capable of self-assembly to form a virus-like particle (VLP), and wherein the peptide antigen is 20 amino acids or less in length and comprises the general sequence: V-X1-T-X2-X3-X4-X5 [SEQ ID NO:96], wherein X1 is E or D; X2 is P or L; X3 is T or I; X4 is R or K, and X5 is N, S or K.