METHOD FOR BIOCATALYTIC SYNTHESIS OF SUBSTITUTED OR UNSUBSTITUTED PHENYLACETIC ACIDS AND KETONES HAVING ENZYMES OF MICROBIAL STYRENE DEGRADATION

摘要

The invention relates to a method for biocatalytic synthesis of substituted and unsubstituted phenylacetic acids and ketones from styrenes and bicyclic aromatic hydrocarbons using enzymes of microbial styrene degradation in a whole-cell sensor, and a kit for said type of biocatalytic synthesis comprising a whole-cell catalyst and novel bacteria stems and to the use thereof in said type of the method, wherein the method comprises the following steps: a) providing at least one type of whole-cell catalyst, comprising genes which are provided for the enzymes of the styrene degradation coding and functionally under the control of a controllable promoter, in an aqueous component, b) activating of the whole-cell catalyst by an inductor and/or an activator leads to expression of the genes, c) contacting the activated whole-cell catalyst to a substrate, d) isolating the formed reaction products, which advantageously are not metabolized further by the whole-cell catalyst and advantageously accumulate in the aqueous component.