REAGENT ALLOWING FOR MICROORGANISM INACTIVATION, EXTRACTION AND STORAGE OF BACTERIAL DNA IN FORM SUITABLE FOR HIGHLY EFFECTIVE MOLECULAR DIAGNOSTICS

摘要

The invention relates to biochemistry, chemistry and medicine field. A reagent for the treatment and storage of a biological sample intended for in vitro diagnostics of infectious disease via PCR method, said reagent being an aqueous solution containing an alcohol, an alkali and a tertiary amine in concentrations providing for amplification efficiency increase for at least 2 cycles, is described. The said reagent provides for neutralization of products contained in biological sample and inhibiting PCR reaction, microorganism inactivation, increase of biological sample shelf life and DNA isolation efficiency.