SYSTEM AND METHOD FOR TIME-RESOLVED FLUORESCENCE IMAGING AND PULSE SHAPING

摘要

A time-resolved fluorescence imaging (TRFI) system that images a target medium without lifetime fitting. Instead of extracting the lifetime precisely, the system images the fluorophore distribution to allow for a simple and accurate method to obtain the fluorescence image without lifetime-extraction for time-resolved fluorescence imaging. An illumination source circuit for TRFI is also disclosed that shapes the excitation pulse. In one embodiment, the illumination source comprises an LED and stub line configured for generating a linear decay profile.

主权项

1. A method for imaging a sample medium, the method comprising;
exciting the sample medium with an excitation light pulse; generating a first image of the medium, said first image comprising data relating to at least a first fluorophore corresponding to a first component of the medium and a second fluorophore corresponding to a second component of the medium, the first fluorophore having a longer fluorescence lifetime than the second fluorophore; generating a second image of the medium at a specified time subsequent to said first image, said second image comprising data relating to at least the first fluorophore; and generating a third image as a function of first image and the second image to identify a contrast between the first component and the second component within the medium.