| 发明名称 |
METHOD FOR REDUCTION OF 1->2 READING FRAME SHIFTS |
| 摘要 |
Herein is reported a method for the recombinant production of a polypeptide, which comprises the dipeptide AR, characterized in that the method comprises the recovering of the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide, whereby the dipeptide AR comprised in the polypeptide is encoded by the oligonucleotide gca cgt, or the oligonucleotide gcg cgt, or the oligonucleotide gcc cgt. |
| 申请公布号 |
US2015056656(A1) |
申请公布日期 |
2015.02.26 |
| 申请号 |
US201414473017 |
申请日期 |
2014.08.29 |
| 申请人 |
Hoffmann-La Roche Inc. |
发明人 |
Grossmann Adelbert;Hesse Friederike;Kopetzki Erhard;Lau Wilma;Schantz Christian |
| 分类号 |
C07K14/775 |
主分类号 |
C07K14/775 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for the reduction of by-product formation by 1->2 frameshift in the production of a polypeptide, which comprises the dipeptide AR (SEQ ID NO: 06), characterized in that the method comprises the following step:
recovering the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide,whereby the oligonucleotide encoding the dipeptide AR comprised in the nucleic acid encoding the polypeptide has the nucleotide ‘c’ at the fourth position. |
| 地址 |
Little Falls NJ US |
