主权项 |
1. A method of providing a genetic profile of a tumor, the method comprising:
providing a sample comprising genomic DNA from a tumor cell; and simultaneously determining the identity of one or more alleles listed in Table 3B for each of EGFR and KRAS plus one or more alleles from one or more of AKT1, APC, BRAF, CTNNB1, FLT3, IDH1, JAK2, KIT, MAP2K1, NOTCH1, NRAS, PIK3CA, PTEN, and TP53 in the genomic DNA, wherein the method comprises determining the identity of each allele using a single base extension reaction, thereby providing a genetic profile of the tumor. |