METHOD FOR DETECTION OF AMYLOID BETA OLIGOMERS IN A FLUID SAMPLE AND USES THEREOF

摘要

The invention herein is directed to a selective A&bgr; oligomer immunoassay capable of reliably and sensitively detecting A&bgr; oligomers in a biological sample of a patient. In one embodiment the inventive assay uses a pair of anti-A&bgr; oligomer antibodies, 19.3 and 82E1, to detect and quantify A&bgr; oligomers in a cerebrospinal fluid (CSF) sample. The inventive assay can be used to differentiate Alzheimer's disease (AD) patients from non-AD patients and/or to stratify AD patients according to the severity of their disease. The inventive assay can also be used as a target engagement assay that can measure bound A&bgr; oligomers as a surrogate end-point for the assessment of therapeutic efficacy and/or target engagement.