METHOD FOR INCREASING THE SECRETION OF RECOMBINANT PROTEINS

摘要

Producing a recombinant protein in cells having a cell wall, comprises increasing the secretion of the recombinant protein by the cell wall through an expression of the protein in the cells in a culture medium containing a combination of a surface active polymer and monovalent metal ions and having an osmolarity of at least 0.32 osmole/l. Independent claims are included for: (1) the culture medium for promoting secretion, comprising 0.05 wt.% of surface active polymer and the metal ions in a concentration of 20 mM; and (2) a dry nutrient mixture medium for reconstitution of the culture medium.