Post protein hydrolysis removal of a potent ribonuclease inhibitor and the enzymatic capture of DNA

摘要

The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.

主权项

1. A method for extracting nicked DNA from a blood sample, the method comprising:
(a) contacting a blood sample containing DNA with an endonuclease or exonuclease to produce a nick in the DNA; (b) contacting the blood sample with a single stranded binding protein (SSB) immobilized on a matrix, whereby the SSB binds to the nick in the DNA to produce an SSB-bound DNA; and (c) extracting the SSB-bound DNA from the blood sample.