Genotypes, alleles and molecular markers associated with asian soybean rust, as well as methods, processes and uses thereof

摘要

The present invention relates to screening methods for rust resistance or tolerance, in particular, Asian soybean rust (ASR—Phakopsora pachyrhizi). In addition, the present invention relates to the use of molecular markers for the Glycine genus, in particular, for the Glycine max species. The present invention further relates to a method for identifying loci with quantitative and/or qualitative traits associated with rust resistance or tolerance in plants by means of molecular markers. Said markers can be used for assisted screening in improvement programs directed to selecting disease-resistant or -tolerant plants. The present invention also relates to gene pyramiding related to rust resistance. The markers of the present invention are also useful for the positional cloning of rust-resistant or -tolerant genes. Also disclosed are a method for obtaining disease-resistant or -tolerant cultivars, a process for obtaining a plant population and a method for controlling diseases in a plant population. Another object of the present invention is the use of species from the Glycine genus as a source of resistance for obtaining ASR-resistant or tolerant cultivars.

主权项

1. A method for identifying and selecting soybean Glycine max individuals carrying the phenotypic trait of resistance and/or tolerance to Asian soybean rust Phakopsora pachyrizi comprising:
a) extracting nucleic acid from a plant of any soybean genotype; b) assaying the extracted nucleic acid for at least one molecular marker mapped at 10 cM or less from the Rpp2 locus to obtain an amplification pattern of at least one molecular marker; c) comparing the amplification pattern obtained in step (b) with an amplification pattern of the Asian soybean rust resistant genotypes PI 224270 or PI 230970, wherein the amplification pattern of the Asian soybean rust resistant genotypes PI 224270 or PI 230970 was obtained using the same at least one molecular marker as that used in step b); and d) selecting plants that exhibit the same amplification pattern as either one of the Asian soybean rust resistant genotypes PI 224270 or PI 230970;
wherein said Rpp2 locus is mapped in linkage group J, flanked by markers Satt215 and Sat—361/Sat—366.