Method for improved fibrin sealing

摘要

The present invention relates to a fibrin matrix, its preparation and use for effectively sealing a defect in a mucosa or other moist tissue.

主权项

1. A method for applying fibrin matrix onto a moist tissue comprising:
a) applying an effective amount of a solid fibrin sealant blend onto the moist tissue, wherein the solid fibrin sealant blend comprises a solid component comprising fibrinogen and a solid component comprising a proteolytic enzyme component which is capable of forming fibrin when it reacts with fibrinogen; and b) applying over at least a portion of the applied solid fibrin sealant blend an effective amount of a liquid fibrin sealant formulation,wherein the effective amounts for solid fibrin sealant blend and liquid fibrin sealant formulation are sufficient to produce a fibrin matrix on the moist tissue having improved sealing strength relative to application of an effective amount of either the liquid fibrin sealant formulation or the solid fibrin sealant blend.