Capping-prone RNA polymerase enzymes and their applications

摘要

The invention provides a chimeric enzyme comprising at least one catalytic domain of a RNA triphosphatase, at least one catalytic domain of a guanylyltransferase, at least one catalytic domain of a N7-guanine methyltransferase, and at least one catalytic domain of a DNA-dependant RNA polymerase. The invention also provides pharmaceutical composition comprising said chimeric enzyme and uses of said chimeric enzyme.

主权项

1. A hetero-oligomeric enzyme comprising:
a first monomer and a second monomer linked together covalently or noncovalently, the first monomer comprising
at least one catalytic domain of a RNA triphosphatase,at least one catalytic domain of a guanylyltransferase, andat least one catalytic domain of a N7-guanine methyltransferase, and the second monomer comprisingat least one catalytic domain of a DNA-dependent RNA polymerase, wherein the hetero-oligomeric enzyme is non-natural.