Interleukin-1α antibodies and methods of use

摘要

Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors.

主权项

1. A method for quantifying the amount of IL-1α in a biological sample, the method comprising the steps of:
(a) separating a biological sample obtained from a human subject using a size-exclusion filter that separates molecules according to molecular weight into a first fraction comprising intact IgG complexed with IL-1α and a second fraction consisting of molecules less than 100 Kda, wherein the biological sample comprises intact IgG complexed with IL-1α, wherein the first fraction does not pass through the filter, and wherein the second fraction passes through the filter; and (b) quantifying the amount of IL-1α in the first fraction.