Combination of fluorescent dyes for the detection of nucleic acids

摘要

The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL.

主权项

1. A composition comprising:
(i) a first nucleic acid to which a blue florescent dye is covalently attached; (ii) a second nucleic acid to which a green fluorescent dye is covalently attached; (iii) a third nucleic acid to which a yellow fluorescent dye is covalently attached, wherein the yellow florescent dye has a relative detection sensitivity in an emission spectrum (peak area of the yellow fluorescent dye:peak area of the blue fluorescent dye) of at least about 77%; and (iv) a fourth nucleic acid to which a red fluorescent dye is covalently attached.