Method for predicting the response of non-small cell lung cancer patients to targeted pharmacotherapy

摘要

The present invention relates to a method for determining or predicting the response of a patient diagnosed with non small cell lung cancer to targeted pharmacotherapy. The present invention also aims to provide methods and devices for predicting the response of patients diagnosed with non small cell lung cancer to specific medicaments. More specifically, the present invention provides methods which measure kinase activity by studying phosphorylation levels and profiles and inhibitions thereof by drugs in samples of said patients.

主权项

1. A method for predicting determining the response of a patient diagnosed with non-small cell lung cancer (NSCLC) to a medicament, comprising the steps of:
(a) providing a solid support having immobilized thereupon at least the 20 protein kinase substrates consisting of SEQ ID NO: 1, 2, 4, 6, 8, 9, 11, 13, 26, 27, 32, 37, 45, 49, 61, 65, 72, 75, 76 and 79; a lysate sample obtained from the NSCLC tumor from said patient; and a medicament; (b) measuring the kinase activity of the lysate sample in the presence and in the absence of said medicament by contacting a first part of said lysate sample with said at least 20 protein kinase substrates in the presence of said medicament, and by contacting a second part of said lysate sample with said at least 20 protein kinase substrates in the absence of said medicament, thereby providing a phosphorylation profile of said lysate sample in contact with and in the absence of said medicament, respectively; and (c) determining from said phosphorylation profiles in contact with and in the absence of said medicament the lysate sample differential phosphorylation level in response to the medicament, said differential phosphorylation level determining the response of said patient to said medicament.