发明名称 |
Perhydrolase variant providing improved specific activity |
摘要 |
An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications. |
申请公布号 |
US8962294(B2) |
申请公布日期 |
2015.02.24 |
申请号 |
US201213657575 |
申请日期 |
2012.10.22 |
申请人 |
E. I. du Pont de Nemours and Company |
发明人 |
Dicosimo Robert;Scott Mark Payne;Gavagan John Edward |
分类号 |
C12N9/18;C12N1/20;C12N15/00;C07H21/04;C12P7/40;D21C9/16;D06L1/04;D06L3/02;D06M16/00;C11D3/386;C11D3/39 |
主分类号 |
C12N9/18 |
代理机构 |
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代理人 |
|
主权项 |
1. An isolated nucleic acid molecule selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide having perhydrolytic activity, said polypeptide comprising the amino acid sequence of SEQ ID NO: 14; (b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 42; and (c) a polynucleotide fully complementary to the polynucleotide of (a) or (b). |
地址 |
Wilmington DE US |