Method of deriving mesenchymal stem cells from ES cells using FGF2

摘要

We disclose a method comprising: (a) providing an embryonic stem (ES) cell; and (b) establishing a progenitor cell line from the embryonic stem cell; in which the progenitor cell line is selected based on its ability to self-renew. Preferably, the method selects against somatic cells based on their inability to self-renew. Preferably, the progenitor cell line is derived or established in the absence of co-culture, preferably in the absence of feeder cells, which preferably selects against embryonic stem cells. Optionally, the method comprises (d) deriving a differentiated cell from the progenitor cell line.

主权项

1. A method of establishing, from an embryonic stem (ES) cell, a mesenchymal progenitor cell line comprising mesenchymal stem cells (MSCs), the method comprising:
(a) propagating an ES cell in the absence of co-culture with a feeder cell layer, in a serum free medium comprising FGF2, (b) selecting mesenchymal stem cells from the propagated cells based on expression of one or more cell surface markers, and (c) growing the mesenchymal stem cells selected in step (b) in medium comprising serum; thereby establishing a mesenchymal progenitor cell line comprising MSCs, wherein the mesenchymal progenitor cell line maintains self-renewal without transformation, and wherein the mesenchymal progenitor cell line is lineage restricted compared to the ES cell.