| 摘要 |
FIELD: biotechnology.SUBSTANCE: bacterium Bacillus subtilis is proposed, producing 5?-aminoimidazole-4-carboxamideriboside (AICAR). The said bacterium comprises deregulated pur-operon against the background of inactivated gene purH, the modified heterologous genes prs and purF E.coli under the control of a strong promoter Pas part of chromosome. At that it has one of the following characteristics: comprises the gene zwf under control of a strong promoter P, comprises the heterologous gene udhA under the control of a strong promoter P, comprises the deletion of the gene sacB. The method of synthesizing AICAR by culturing the said bacteria under proper conditions is also proposed. At that culturing is carried out on the medium of the following composition, wt %: fodder yeast 0.5-1.0, sucrose 10-13, soy isolate 2.5-3.5, corn-steep extract 3.0-5.0, urea 0,6-0,8, (H3)3CO0.8-1.6, propinol 0.4-0.5, water - the rest.EFFECT: high level of synthesis of AICAR.5 cl, 2 tbl, 9 ex |
