COMPOSITIONS AND METHODS FOR MODULATING RNA

摘要

Aspects of the invention relate to methods for increasing gene expression in a targeted manner. In some embodiments, methods and compositions are provided that are useful for posttranscriptionally altering protein and/or RNA levels in a targeted manner. Aspects of the invention disclosed herein provide methods and compositions that are useful for protecting RNAs from degradation (e.g., exonuclease mediated degradation).

主权项

1. A method of increasing gene expression in a cell, the method comprising:
delivering to a cell an oligonucleotide comprising the general formula 5′-X1—X2-3′, wherein X1 comprises 5 to 20 nucleotides that have a region of complementarity that is complementary with at least 5 contiguous nucleotides of an RNA transcript encoded by the gene, wherein the nucleotide at the 3′-end of the region of complementary of X1 is complementary with the nucleotide at the transcription start site of the RNA transcript; and X2 comprises 1 to 20 nucleotides.