METHOD OF DETECTING TARGET MATERIAL, SENSOR CHIP, AND DETECTING DEVICE

摘要

A method of detecting a target material (3) includes: a step of preparing a complex (11), the complex including an aptamer (1) to which a target material (3) in a specimen specifically binds, a first nucleic acid fragment (2) that has a base sequence complementary to the aptamer (1), and a fixing member (4) to which a part of the aptamer (1) and a part of the first nucleic acid fragment (2) are fixed, in which the aptamer (1) has a double strand-forming site (5) capable of forming a double strand with the first nucleic acid fragment (2); a step of separating the first nucleic acid fragment 92) from the double strand-forming site (5) of the aptamer (1) by binding the target material (3) to the aptamer (1); and a step of detecting the cleavage of the double strand.

主权项

1. A method of detecting a target material, the method comprising:
a step of preparing a complex, the complex including an aptamer to which a target material in a specimen specifically binds, a first nucleic acid fragment that has a base sequence complementary to the aptamer, and a fixing member to which a part of the aptamer and a part of the first nucleic acid fragment are fixed, in which the aptamer has a double strand-forming site capable of forming a double strand with the first nucleic acid fragment; a step of separating the first nucleic acid fragment from the double strand-forming site of the aptamer by binding the target material to the aptamer; and a step of detecting the cleavage of the double strand where the first nucleic acid fragment is separated from the aptamer.