发明名称 |
RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
摘要 |
Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit. |
申请公布号 |
US2015050699(A1) |
申请公布日期 |
2015.02.19 |
申请号 |
US201314385857 |
申请日期 |
2013.03.20 |
申请人 |
VILNIUS UNIVERSITY |
发明人 |
Siksnys Virginijus;Gasiunas Giedrius;Karvelis Tautvydad;Lubys Arvydas;Zaliauskiene Lolita;Glemzaite Monika;Smith Anja |
分类号 |
C12N15/90;C12N9/22;C12N15/113;C12N15/10 |
主分类号 |
C12N15/90 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Vilnius LT |