METHODS OF PERFORMING A SIZING ANALYSIS USING A CORRECTED SIZING LADDER

摘要

The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.

主权项

1. A method of performing a sizing analysis, the method comprising:
providing an archival sizing ladder, the archival sizing ladder including previously measured archival ladder migration times; loading a separation channel of a fractionation device with a sample containing a low standard marker; separating the sample into a plurality of separated components; detecting the low standard marker, thereby providing a low standard marker migration time; aligning the low standard marker migration time with the corresponding archival sizing ladder migration time, thereby providing a corrected archival sizing ladder; and performing a sizing analysis of the separated components using the corrected archival sizing ladder in comparison with a parameter for at least one of the separated components, the parameter being selected from the group consisting of separated component migration time and separated component band position.